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Microscopy

About us

The Microscopy Unit at the Veterinary Centre for Resistance Research (TZR) offers a collection of state-of-the-art light microscopy equipment to support anti-infective research.

We provide comprehensive technical and scientific support as well as user training for all microscopy systems to perform sophisticated imaging experiments and generate high-quality image data from a wide variety of samples ranging from bacteria to cells to tissues.

Available microscopy techniques include:

  • widefield fluorescence microscopy
  • confocal laser scanning microscopy
  • fluorescence lifetime microscopy (FLIM)
  • fluorescence lifetime correlation spectroscopy (FLCS)

As part of collaborative projects, we offer expertise and service in design and standardization of imaging experiments as well as prior specimen preparation. In addition, we establish image processing routines and automated image analysis workflows using classic computer vision as well as machine learning-based methods.

Instruments

Widefield – Leica DMi8 inverse

Widefield system suited for imaging of multicolour fixed fluorescently and histologically stained samples.

CLSM – Leica Stellaris 8 FALCON

Inverted confocal laser scanning microscope equipped with white light laser (440-790 nm), 405 nm diode laser, 6 detectors (3 Power HyD S, 1 Power HyD X, 1 Power HyD R, 1 transmitted light channel (BF)), resonant scanner, FAst Lifetime CONtrast (FALCON), Lightning, Hamamatsu Flash 4.0 sCMOS camera, environmental control.

Image Processing Workstation

Mid-level HP Z4G workstation equipped with 6 cores (Intel Xenon W-2133), 64 GB RAM, Nvidia Quatro RTX 5000 running LAS X offline software and LAS X Lightning Process.

Contact

Dr. Andreas Nerlich

Veterinary Center for Resistance Research (TZR)

-Head Microscopy Unit-

Department of Veterinary Medicine

Freie Universität Berlin

Robert-von-Ostertagstr. 8

D-14163 Berlin, Germany

Tel.: +49 30 838 58508

Fax.: +49 30 838 458508

E-mail: andreas.nerlich@fu-berlin.de

Booking

Direct link to book services and equipment viaOpenIRIS.