Ruth Leben, Ph.D. in Biomedical Sciences
Institut für Immunologie - AG Hartmann
und Deutsches Rheuma-Forschungszentrum (DRFZ) - AG Niesner
Wissenschaftliche Mitarbeiterin (Drittmittel)
- Movement of nematodes in the mouse gut using two-photon microscopy and fluorescence lifetime imaging (FLIM) as a method for imaging host and parasite metabolism and monitoring the mucus viscosity.
- Intravital multi-photon microscopy, in particular FLIM of the metabolic enzyme NAD(P)H and the FRET construct TN-XXL.
- Development of an algorithm based on the phasor approach to analyze and map metabolic enzyme activity using NAD(P)H-FLIM.
- Calculation and mapping of the absolute intercellular calcium concentration using vector analysis of the TN-XXL donor fluorescence lifetime.
- Two-photon excitation spectra of various fluorescent proteins.