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Is it possible to use the same settings for Analyser and Sorter?

In general, yes, because the same software is used on both devices. However, the Analyser is more sensitive than the Sorter. Moreover, distinguishing the cell populations using different fluorochromes is more important when working with the Sorter compared to the Analyzer. It is helpful to test different sorting strategies to find out what works best.

What works with the Analyser does not need to get good results using the Sorter!