Methods

Plastination

To give students the opportunity to develop a multidimensional understanding of the body outside of the dissecting room and regardless of place and time, we have introduced plastination at the institute. These technique produces non-perishable specimens that are aesthetically pleasing, very realistic, durable as well as odor-free, are not affected by light or higher temperatures and do not present any health hazard (pregnant students). Our collection is continually expanded.


     

 

Morphology

Anatomical-histological examination methods including various ways of fixating, embedding and staining

Cytometry, morphometry, fluorescence microscopy, fluorometry

Transmission electron microscopy of organs and tissues as well as of two- and three-dimensional cell cultures

Direct and indirect immunhostochemistry and cytochemistry, glycohistochemistry

Various methods to verify apoptosis

 

           

 

Cell biology

Isolation, identification and characterization of numerous cell types (e.g. microvascular endothelial cells, stem cells from the yolk sac, adult stem cells, ganulosa cells, tumor cells etc.)

Establishment of in vitro models of angiogenesis and vasculogenesis

Image Analysis

Life cell imaging

Proliferation tests 


 

    

 

Molecular biology

Genomic

Isolation of DNA and RNA from various cells

PCR, RT-PCR, real time RT-PCR

In situ-Hybridisation

Transfection of various cells in vitro by means of biological transfection methods

Gene silencing

Proteomics

Isolation of proteins from tissue and cell cutures

SDS-Page

Western Blot

2 D-elektrophoresis