Pathogens in swine threaten the economical basis of food producers as well as the health of costumers. Food is the most common carrier of zoonotic diseases.
Our goal is to develop a DNA-chip based System for the parallel detection of the most common zoonotic pathogens in swine within the whole food-chain. In the main focus, we are working on the application specific development of a ligase dependend Simplex PCR (LDA-Simplex-PCR) to be able to detect the sequences of the most common zoonotic pathogens that we are interested in.
The PorkChip detection is devided in four steps:
(1) Cultural augmentation (optional, if needed)
(2) DNA isolation
(3a) Ligase dependend amlification and modification of the target sequences
(3b) Amlification of the LDA-generated samples via Simplex-PCR
(4) Detection via DNA-chip
Because of the different and special knowledge needed for the whole project, we did a seperation in three special compartments. The division into three differents groups enables us to have the best knowledge and cognition concentrated in each group.
TP1 DNA isolation / LDA / Simplex-PCR (CONGEN)
TP2 Real-Time PCR / PCR / Microbiology (IMT)
TP3 Bioinformatics / Chipdevelopment (IBMT)
Brucella melitensis biovar suis
Brucella melitensis biovar abortus
Brucella melitensis biovar melitensis