Pork chip


Development of a system to detect economically and sanitary relevant pathogens in swine

Pathogens in swine threaten the economical basis of food producers as well as the health of costumers. Food is the most common carrier of zoonotic diseases.
Our goal is to develop a DNA-chip based System for the parallel detection of the most common zoonotic pathogens in swine within the whole food-chain. In the main focus, we are working on the application specific development of a ligase dependend Simplex PCR (LDA-Simplex-PCR) to be able to detect the sequences of the most common zoonotic pathogens that we are interested in.

The PorkChip detection is devided in four steps:

(1)       Cultural augmentation (optional, if needed)
(2)       DNA isolation
(3a)     Ligase dependend amlification and modification of the target sequences
(3b)     Amlification of the LDA-generated samples via Simplex-PCR
(4)       Detection via DNA-chip

Because of the different and special knowledge needed for the whole project, we did a seperation in three special compartments. The division into three differents groups enables us to have the best knowledge and cognition concentrated in each group.

TP1     DNA isolation / LDA / Simplex-PCR (CONGEN)
TP2     Real-Time PCR / PCR / Microbiology (IMT)
TP3     Bioinformatics / Chipdevelopment (IBMT)

Selected Pathogens:

- Bacteria
Brucella melitensis biovar suis
Brucella melitensis
biovar abortus
Brucella melitensis
biovar melitensis
Campylobacter coli   
Campylobacter jejuni           
Listeria monocytogenes
Salmonella typhimurium
Mycobacterium tuberculosis
Mycobacterium bovis
Yersinia enterocolitica
Clostridium perfringens
Streptococcus suis
Bacillus anthracis
Clostridium botulinum

Toxoplasma gondii
Trichinella spiralis



Dr. Sebastian Günther, Birgit Lauzat

Grants / Funding:

- Bundesministerium für Bildung und Forschung (BMBF)
- BioProfil Nutrigenomik

Collaboration with:

- CONGEN Biotechnologie GmbH (Congen)
- Fraunhofer Institut für Biomedizinische Technik (IBMT)