In this project founded by the Bundesministerium für Bildung und Forschung (BMBF) foodborne zoonotic infections will be examined by 16 different project partners originating from veterinary as well as human medicine focussing on important aspects of foodborne infections with Salmonella spp., Campylobacter spp., Yersinia spp. und shiga-toxin producing Escherichia coli (STEC).
At the “Institut für Mikrobiologie und Tierseuchen” two individual projects will be processed. While the individual project “Determinants of host-specific pathogenesis and persistence of Salmonella serovars in animals and man” is performed by the research group "Intestinal Cellular Microbiology" (ICM) our research group deals with DNA sequence-based typing of Shiga toxin-producing Escherichia (E.) coli and studies on zoonotic pathogen host-specificity in a chicken infection model.
Enterohemorrhagic E. coli (EHEC), a subgroup of shiga toxin (Stx)-producing E. coli (STEC) are not only responsible for severe enteric diseases like hemorrhagic colitis but also for extragastrointestinal sequelae like hemolytic uremic disease (HUS). Next to the clinical important STEC-serovar O157:H7 several non-O157, in particular serovars O26, O96; O111, O103 and O145, emerged as important pathogens that are associated with human diseases.
To reveal epidemiological associations typing of isolates is of utmost importance. The world wide gold standard for phenotypical E. coli typing is serotyping. Because this conventional method has serious drawbacks newer and molecular based methods have been introduced in epidemiological typing of E. coli as well as for several other pathogens.
Therefore we will examine a large number of non O157-STEC isolates applying DNA-based methods like multi-locus sequence typing (MLST) and single-locus sequence typing (SLST) of the eae and nleA gen. Based on results of MLST and SLST analyses together with epidemiological data analyses single nucleotide polymorphisms (SNP) will be ruled out as diagnostic markers being specific for the most virulent non-157 STEC`s.
Projekt Food-Borne Zoonotic Infectons of Humans (FBI-Zoo), funded by the Bundesministerium für Bildung und Forschung (BMBF)